Solution
ExoCargo
Exosome Drug Loading and Delivery Development Services
ExoCargo is DrSignal Biotechnology's exosome drug loading and targeted delivery development service, helping clients encapsulate small-molecule drugs, nucleic acids or peptides into exosomes and apply surface targeting modifications. The service covers loading condition screening, encapsulation process development, in vitro release and stability validation through small-scale trial production, carried out in closed tubing and an ISO Class 7 environment, with batch-to-batch variation in loading efficiency and particle size distribution kept within 15%.
Cargo Loading & Encapsulation
Exosome Drug Loading and Encapsulation
Depending on the drug molecule's properties, loading is performed by electroporation, co-incubation or saponin permeabilization, with loading efficiency and integrity assessed by nanoparticle tracking analysis and fluorescence quantification. After encapsulation, free drug is removed by tangential flow filtration in a closed loop, keeping the free fraction below 5%.
Surface Targeting Modification
Surface Targeting Modification
Targeting peptides or antibody fragments are conjugated to the exosome surface, using lipid anchoring or genetic engineering to build a delivery vehicle directed at specific tissues or cells, with target-binding specificity confirmed by bead-based flow cytometry and confocal microscopy.
Manufacturing Process
From indication assessment to technology transfer, the service runs through eight stages. Every batch carries a unique development number bound to its experimental records, and clients can check loading data, release curves and stability reports through a dedicated account.
Indication and Target Assessment
Exosome Source Selection
Loading Condition Optimization
Encapsulation and Purification
Surface Modification
In Vitro Release Testing
Stability and Activity Validation
Technology Transfer and Pilot Production
Technical Advantages
Multi-Strategy Drug Loading Platform
Supports multiple loading routes including electroporation, co-incubation, saponin permeabilization and lipid fusion, with the optimal strategy selected according to the properties of different cargoes such as small molecules, siRNA, mRNA or peptides.
Quantifiable Loading and Release Analysis
Loading amount, encapsulation rate and in vitro release curves are measured in parallel by nanoparticle tracking analysis, fluorescence quantification and HPLC, with batch-to-batch variation across three validation batches all below 15%.
Targeting Peptide Surface Engineering
Targeting ligands are displayed on the exosome surface via lipid-anchored peptides and genetic engineering display technology, with directing specificity verified by competitive binding assays to reduce off-target tissue distribution.
ExoCargo Development Service Guide
PDFCovers loading strategies, targeting modification options, analytical methods and the collaborative development process.